Pasteurella multocida B:2 ve B:3,4 Serotiplerinin Ortak İmmunojenik Protein Komponentlerinin Karşılaştırılması

Serotype B of Pasteurella multocida is a major agent of haemorrhagic septicaemia (HS), a form of blood poisoning widespread in cattle and buffalo from Southern Asia. The vaccines currently used against the disease are not potent enough, being effective only for a limited duration only. In an attempt to identify immunogenic components of P. multocida for use as live/subunit vaccines, the outer membrane proteins (OMPs) of B:2 and B:3,4 serotypes were isolated and their profiles compared. Three common immunogenic components; polypeptides of molecular weight (MW) 39.0, 33.5, 31.0 kDa, were identified by immunoblotting. The immunogenicity of identified OMPs was also confirmed using antisera generated against them. The antigenic OMPs identified in current research could be explored in the formulation of new, safe and effective vaccines for indigenous buffalo and cattle breeds with a prolonged efficacy.